Abstract:
There are numerous medicinal plants in the region of djelfa in Algeria, including Peganum harmala, Artemisia campestris, Opuntia, and Picris hieracioides. Traditional medicine employs these plants due to their medicinal properties. Algeria's diverse plant flora contains numerous medicinal plants, such as Peganum harmala, Artemisia campestris, Opuntia, and Picris hieracioides. Traditional medicine has utilized these plants for their therapeutic potential. High yields of organic extracts were obtained from Peganum harmala leaf extract and Artemisia campestris roots. Utilizing the Folin-Ciocalteu method, the total phenolic content was determined. The concentration was highest in the aerial portion of Artemisia campestris (435.86 mg EAC/g of extract), followed by the root portion (323.69 mg EAC/g of extract). The AlCl3 method was used to determine flavonoid content, with assay results ranging from 95.70 to 2.60 mg EQ/g of extract. The leaf extract of Picric hieracioides exhibited the highest antiradical activity at 45.07%, surpassing all other extracts. Overall, however, the antioxidant capacity was only moderate. Using disk diffusion assays, antimicrobial activity was evaluated against six bacterial strains. The extracts showed sensitivity against all tested microorganisms, but Opuntia extract did not show any effect on Streptococcus pneumonia, Pseudomonas aeruginosa, or Staphylococcus aureus.
Description:
Nowadays, a large number of aromatic and medicinal plants have very important biological properties which find many applications in various fields, namely in medicine, pharmacy, cosmetology and agriculture. This renewed interest comes on the one hand from the fact that medicinal plants represent an inexhaustible source of bioactive substances, and on the other hand, the side effects induced by the drugs worry the users who turn to less aggressive care for the body. In the present work, different aspects have been studied some photochemical properties and antioxidant and antimicrobial activities of crude extracts.
The extraction of the aerial and root part of these plants has made it possible to obtain yields which differ according to these plants used and the method of extraction, therefore the content of phenolic compounds, flavonoids are relatively different.
The antioxidant activity of the different extracts was evaluated by the DPPH free radical reduction method. In the DPPH free radical reduction test, the scavenging activity is high in the polar extract.
The antimicrobial activity was determined on six bacterial strains, according to the disk diffusion method. The results indicate that the extracts have antimicrobial activity on all the strains tested except the opuntia extract, which has no activity on the strains: Streptococcus pneumonia, Pseudomonas aeruginosa, and Staphylococcus aureus. Following these results, it would therefore be interesting to extend the range of antioxidant and antimicrobial tests as well as the isolation and characterization of the active compounds in the different extracts in order to identify the different molecules responsible for the different biological activities. of these plants.
All of these results obtained in vitro constitute only a first step in the search for biologically active substances of natural origin, an in vivo study is desirable, to obtain a more in-depth view of the antioxidant and antimicrobial activities of the extracts of these plants.
